rabbit polyclonal antibodies targeting scavenger receptor class b type i sr bi (Novus Biologicals)
Structured Review

Rabbit Polyclonal Antibodies Targeting Scavenger Receptor Class B Type I Sr Bi, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibodies targeting scavenger receptor class b type i sr bi/product/Novus Biologicals
Average 95 stars, based on 266 article reviews
Images
1) Product Images from "HDL inhibits the effects of oxidized phospholipids on endothelial cell gene expression via multiple mechanisms"
Article Title: HDL inhibits the effects of oxidized phospholipids on endothelial cell gene expression via multiple mechanisms
Journal: Journal of Lipid Research
doi: 10.1194/jlr.m047738
Figure Legend Snippet: Fig. 2. The effect of HDL on OxPAPC signaling is not mediated by SR-BI. HAECs were transfected with either siRNA targeting SR-BI or scramble con- trol siRNA and treated 48 h later with media, Ox- PAPC (50 g/ml), HDL (50 g/ml), or OxPAPC and HDL (50 g/ml) as described in the Materials and Methods. The mRNA expression levels of KLF2 , ATF3 , INSIG1 , IL8 , and HO1 were determined by qPCR and normalized to GAPDH expression levels. All data are presented as average log2-fold change of triplicates ± SEM. Asterisks indicate signifi cance level in unpaired Student’s t -test (* P < 0.05, ** P < 0.01, *** P < 0.001) A: Despite effective silencing of SR-BI, the expression patterns of KLF2 , ATF3 , LDLR , and IL-8 after OxPAPC or OxPAPC+HDL treatment were unchanged. Results were confi rmed using two separate siRNAs. B: Similarly, blocking of SR-BI us- ing SR-BI antiserum (1:300 dilution) had no effect on the HDL response of KLF2 , ATF3 , LDLR , or IL-8 . Rabbit IgG was used as control. C: HAECs were ei- ther untreated or treated with OxPAPC (50 g/ml) in the presence or absence of HDL (50 g/ml) or delipidated apoA1 (25–50 g/ml). HDL and apoA1 were added for 1 h of pretreatment and 4 h of cotreatment. The mRNA expression levels of KLF2 , ATF3 , INSIG1 , IL-8 , and HO1 were determined by qPCR and normalized to GAPDH expression levels.
Techniques Used: Transfection, Expressing, Blocking Assay, Control